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 Table of Contents  
Year : 2019  |  Volume : 9  |  Issue : 1  |  Page : 32-34

Possible acute rejection associated with the use of the new antihepatitis C virus medications

1 Department of Internal Medicine, Tawam Hospital, AlAin, United Arab Emirates
2 Division of Nephrology, Tawam Hospital, AlAin, United Arab Emirates
3 Department of Pathology, College of Medicine and Health Sciences, UAE University, AlAin, United Arab Emirates

Date of Web Publication4-Jan-2019

Correspondence Address:
Dr. Khaled A Karkout
Department of Internal Medicine, Tawam Hospital, AlAin
United Arab Emirates
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/AJM.AJM_171_17

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Hepatitis C virus infection is associated with increased morbidity and mortality. It remains a major challenge for management and treatment, especially in patients with renal transplant. The new directacting antiviral agents gave big hopes to both clinicians and patients that they can overcome this challenge without major side effects. Studies recently have supported this claim; however, they are still few, limited, and may give false hopes. In the following case report, we present a case, supported by histological evidence about a possible acute rejection of kidney transplant after treatment with these new medications. This case is limited by the absence of donorspecific antibodies. This report is aimed to increase awareness about the urgent need for further studies.

Keywords: Acute rejection, anti-hepatitis C virus medication, daclatasvir, direct-acting antiviral agents, kidney transplant, sofosbuvir

How to cite this article:
Karkout KA, Al Sherif S, Hussein Q, Albawardi A, Boobes Y. Possible acute rejection associated with the use of the new antihepatitis C virus medications. Avicenna J Med 2019;9:32-4

How to cite this URL:
Karkout KA, Al Sherif S, Hussein Q, Albawardi A, Boobes Y. Possible acute rejection associated with the use of the new antihepatitis C virus medications. Avicenna J Med [serial online] 2019 [cited 2019 May 24];9:32-4. Available from: http://www.avicennajmed.com/text.asp?2019/9/1/32/245372

   Introduction Top

Hepatitis C virus (HCV) infection is prevalent in renal allograft recipient and associated with increased morbidity and mortality. The new directacting antiviral agents (DAAs) are highly effective in clearing the virus and considered safe for use in kidney transplant (KT) patients; the early studies did not report acute graft rejection after their use. Here, we are reporting a case of biopsyproven acute graft rejection after the use of DAAs.

   Case report Top

It describes the patient: Male, 47 years old, had a living unrelated kidney transplant outside UAE. Before his transplant, he acquired HCV infection. In 2009, he was found to have 12 million copies/ml of HCV (genotype one) with a deranged liver function test. In 2015, the patient showed F3 fibrosis on FibroScan. However, due to the risk of graft rejection, he was not started on intraneural facilitation therapy and kept on low immunosuppressive treatment consisting of cyclosporine (CYC) 75/50 mg daily, mycophenolic acid 180 mg twice daily, and prednisolone 5 mg daily. Serum creatinine (Cr) level was maintained mostly around 1.4–1.5 mg/dL. Recently, DAAs became available, and he was started on daclatasvir (DAC) 60 mg daily with sofosbuvir (SOF) 400 mg daily. About 3 months after initiating antiHCV treatment, his Cr raised to 2.4 mg/dL and kept rising steadily reaching 4.78 mg/dL. During the course of DAA treatment, CYC trough level was kept always therapeutic (140–180 ng/mL), and a kidney biopsy was done almost 3 month after starting DAAs (10 years and 6 months posttransplant) and revealed features in keeping with acute/active antibody-mediated rejection (AMR). The biopsy showed diffuse glomerulosclerosis consistent with advanced diabetic neohropathy, acute tubual necrosis, severe interstitial fibrosis and tubular atrophy, and borderline changes suspicious for acute T-cell-mediated rejection Figure 1] - Banff scores (t1, i2, g1, ci3, ct3, v0, cv2, ah3, ptc2, mm3, and C4d1), there was no evidence of chronic AMR, and CMV and adenovirus tests were negative. As mentioned above, the Findings were suggestive of an acute AMR; however,we could not confirm it by doing donor-specific antibodies (DSAs) in serum due to the unavailability of the tissue typing of the donor, hence, the possibility of advanced diabetic nephropathy and severe interstitial fibrosis couldn’t be completely eliminated Banff scores (t1, i2, g1, ci3, ct3, v0, cv2, ah3, ptc2, mm3, and C4d1). There is no evidence of chronic AMR, CMV, and adenovirus negative. Findings are suggestive of acute AMR; however, we could not confirm it by doing donorspecific antibodies (DSAs) in serum as we do not have the tissue typing of the donor. AMR as well as features of advanced diabetic nephropathy and severe interstitial fibrosis and tubular atrophy. He received methylprednisolone intravenous pulses, and CYC was replaced by tacrolimus together with increasing the dose of mycophenolic acid. The AMR responded partially with the gradual improvement of his Cr [Figure 2].
Figure 1: (a) Lowpower view of renal biopsy reveals advanced chronic injury (Masson’s trichrome stain, ×4). (b) One glomerulus displays ischemic changes in the form of thickening and wrinkling of glomerular capillary basement membranes and another (star) displays focal segmental glomerular sclerosis. Severe arteriolar hyaline (arrow) is evident (periodic acid–Schiff stain, ×20). (c) Mildtomoderate mesangial matrix expansion without significant hypercellularity (periodic acid–Schiff stain, ×40). (d) Acute tubular injury and multifocal nuclear regenerative atypia (arrow) are identified, and polyomavirus, cytomegalovirus, and adenovirus immunostains were negative (H and E, ×20). (e) Prominent peritubular capillary margination by inflammatory cells (red arrow, H and E, ×40). (f) Peritubular capillary stain positive using C4d immunostain (black arrow, ×40)

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Figure 2: Creatinine level changes

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   Discussion Top

It is well known that HCV infection in renal transplant patients significantly affects the overall survival and graft survival.[1] Interferon therapy, which used to be the backbone of HCV infection treatment, is relatively contraindicated in renal transplant patients due to the high risk of acute transplant rejection.[2],[3]Recently, the introduction of DAAs made it possible to treat HCV infection in solid organ recipients effectively and safely. The early published data did not report acute transplant rejection in patients with DAAs. DAC and SOF, which were used in our patient, are approved for genotypes one to three. Many studies and reports showed that they are effective and safe when they are used in solid organ recipients. DAC is a NS5A inhibitor that does not require dose adjustments with CYC and tacrolimus coadministration.[4] Schrezenmeier etal.[5] studied the pharmacokinetics of DAC and SOF in a prospective cohort of 16 HCVpositive KT recipients; all had estimated glomerular filtration rate (GFR) >30 ml/min. They found that the administration of DAC/SOF is safe and highly efficient in KTR. There was no dose accumulation of DAC and SOF with GFR between 30 and 60 ml/min).[5]

Kamar et al.[6] reported that a significant decrease in calcineurin inhibitor levels was observed after HCV clearance from 25 KT recipients who received SOF‐based therapy. Xue etal.[7] published the results of using DAC/SOF to treat six consecutive Chinese KT patients. They found them highly efficient and safe.[7] Another abstract suggested that the calcineurin levels should be carefully monitored for patients on tacrolimus as it increases or decreases significantly after the initiation of SOF requiring dose adjustments.[8] A recently published metaanalysis evaluating the efficacy and safety of SOF‐based therapies for hepatitis C in liver transplant (LT) recipients (22 studies/1730 recipients) found that SOF‐based therapy is an effective and well‐tolerated treatment strategy for patients with HCV infection recurrence after liver transplantation (Qu Y., 2017). The safety and efficacy of this regimen were reported initially in one case report of a LT patient.[9] Saxena etal.[10] found that in HCVTARGET study, using DAAs (mainly SOFbased regimens) to treat HCV in transplant recipients (347 LT recipients, 60 KT recipients, and 36 dual liverkidney transplant recipients) is safe and effective. However, they reported two cases of kidney rejection during treatment (one on SOF + LDV and the other on LDV + SOF + ribavirin [RBV]).[10] Another case published in 2015 reported HCV eradication in a KT patient using SOF and simeprevir with an excellent safety profile.[11] Another French study published recently looked at 333 patients with HCV genotype 3. It lasted for 24 weeks and involved patients with recurrence of HCV infection postLT and those with an indication for renal or LT. The study concluded that the combination of SOF/DAC with or without RBV has an excellent safety profile in regard to kidney function test.[12] However, taking in consideration, the lack of trials, and previous reports of welltolerated response to the new antiHCV meds in transplant patients, our case would be the first of kind to report acute rejection after their use. Another case that we came across in our hospital reacted in the same way to the combination of SOF and DAC, but unfortunately, there was no biopsy taken, and hence, we reported only our case that is supported by histological evidence. In 2015, an abstract presented in the journal of the American Society of Nephrology described the occurrence of antineutrophil cytoplasmic antibodyassociated rapid progressive glomerulonephritis, after initiation of SOF with RBV.[13]

   Conclusion Top

in our patient, the use of DAAs was associated with what seems to be an acute AMR. However, despite the availability of a histological evidence, the possibility of a chronic rejection still stands in the light of absent DSAs which was not available in our report. This report aims at raising the need to be more cautious when using these medications in KT patients. More studies are needed to establish the link between this treatment and graft rejection.

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Conflicts of interest

There are no conflicts of interest.

   References Top

BaidAgrawal S, Pascual M, Moradpour D, Frei U,TolkoffRubin N. Hepatitis C virus infection in haemodialysis and kidney transplant patients. Rev Med Virol 2008;18:97-115.  Back to cited text no. 1
Kidney Disease: Improving Global Outcomes. Kidney Disease: Improving Global Outcomes clinical practice guidelines for the prevention, diagnosis, evaluation, and treatment of hepatitis C in chronic kidney disease. Kidney Int 2008;73:S199.  Back to cited text no. 2
Wéclawiack H, Kamar N, Mehrenberger M, GuilbeauFrugier C, Modesto A, Izopet J, et al. Alphainterferon therapy for chronic hepatitis C may induce acute allograft rejection in kidney transplant patients with failed allografts. Nephrol Dial Transplant 2008;23:1043-7.  Back to cited text no. 3
Tischer S, Fontana RJ. Drugdrug interactions with oral antiHCV agents and idiosyncratic hepatotoxicity in the liver transplant setting. J Hepatol 2014;60:872-84.  Back to cited text no. 4
Schrezenmeier E, Galander P, Hoffmann F, Jaeger C, Lisec J, Schrezenmeier J, et al. Pharmakokinetics of daclatasvir, sofosbuvir and GS331007 in a prospective cohort of HCV positive kidney transplant recipients. Am J Transplant 2017;17 Suppl 3.  Back to cited text no. 5
Kamar N, Marion O, Rostaing L, Cointault O, Ribes D, Lavayssière L, et al. Efficacy and safety of sofosbuvirbased antiviral therapy to treat hepatitis C virus infection after kidney transplantation. Am J Transplant 2016;16:14749.  Back to cited text no. 6
Xue Y, Zhang LX, Wang L, Li T, Qu YD, Liu F, et al. Efficacy and safety of sofosbuvir and daclatasvir in treatment of kidney transplantation recipients with hepatitis C virus infection. World J Gastroenterol 2017;23:596976.  Back to cited text no. 7
Horn KS, Martin MT, Tang IY. Effectiveness of simeprevir and sofosbuvir in the treatment of hepatitis C virus in genotype 1 postkidney transplant recipients. American Society of Nephrology 2017.  Back to cited text no. 8
Qu Y, Guo Y, Li T, Ye Q, Sun C, Wang L, et al. Efficacy and safety of sofosbuvir-based interferon-free therapies for hepatitis C in liver transplant recipients. Journal of gastroenterology and hepatology 2017;32:740-8.  Back to cited text no. 9
Saxena V, Koraishy FM, Sise M, Lim JK, Chung RT, Liapakis A, et al. LP08: Safety and efficacy of sofosbuvircontaining regimens in hepatitis C infected patients with reduced renal function: Realworld experience from HCVtarget. J Hepatol 2015;62:S263864.  Back to cited text no. 10
Bonacci M, Londoño MC, Esforzado N, Forns X, Sotoca JM, Campistol JM, et al. Antiviral treatment with sofosbuvir and simeprevir in a kidney transplant recipient with HCVdecompensated cirrhosis: Viral eradication and removal from the liver transplant waiting list. Transpl Int 2015;28:13459.  Back to cited text no. 11
Hézode C, Lebray P, De Ledinghen V, Zoulim F, Di Martino V, Boyer N, et al. Daclatasvir plus sofosbuvir, with or without ribavirin, for hepatitis C virus genotype 3 in a french early access programme. Liver Int 2017;37:131424.  Back to cited text no. 12
Gadde S. Antineutrophil cytoplasmic antibody crescentic allograft. World J Nephrol 2016;5:547-50.  Back to cited text no. 13


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